| Primary Identifier | MGI:5000222 | Allele Type | Targeted |
| Attribute String | Modified isoform(s) | Gene | Rbck1 |
| Transmission | Germline | Strain of Origin | (C57BL/6NCrlj x CBA/JNCrlj)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exons 7 and 8 were replaced with a neomycin selection cassette. The absence of protein expression was confirmed by western blot analysis on liver extracts using antibody to the C-terminal part of the protein. Subsequent studies showed that an N-terminal portion of the protein containing the UBL domain was still present, possibly the product of a splice variant of the gene not affected by the mutation, or because of incomplete nonsense-mediated mRNA decay (NMD). |
