| First Author | Gicheva N | Year | 2016 |
| Journal | Biochem Biophys Res Commun | Volume | 479 |
| Issue | 1 | Pages | 1-4 |
| PubMed ID | 27524237 | Mgi Jnum | J:238953 |
| Mgi Id | MGI:5824621 | Doi | 10.1016/j.bbrc.2016.08.055 |
| Citation | Gicheva N, et al. (2016) Siglec-F is a novel intestinal M cell marker. Biochem Biophys Res Commun 479(1):1-4 |
| abstractText | Intestinal microfold (M) cells are epithelial cells primarily present on Peyer's patches (PPs) in the small intestine. The ability of M cells to shuttle antigens into the PP for appropriate immune responses makes M cells a target for next-generation oral vaccine delivery. In this regard, discovery of M cell-specific receptors are of great interest, which could act as molecular tags for targeted delivery of cargo to M cells. Here, using a monoclonal antibody we generated to the Sialic acid-binding immunoglobulin-like lectin F (Siglec-F), we show that Siglec-F is expressed on mouse M cells in the small intestine. Immunohistochemical analysis of the PP tissue sections shows that Siglec-F is expressed on the surface of the M cell membrane exposed to the intestinal lumen. Anti-Siglec-F antibody injected into the mouse small intestine bound to M cells, demonstrating the potential to target M cells via Siglec-F. |
