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Publication : Automated and rapid self-report of nociception in transgenic mice.

First Author  Black CJ Year  2020
Journal  Sci Rep Volume  10
Issue  1 Pages  13215
PubMed ID  32764714 Mgi Jnum  J:299246
Mgi Id  MGI:6457746 Doi  10.1038/s41598-020-70028-8
Citation  Black CJ, et al. (2020) Automated and rapid self-report of nociception in transgenic mice. Sci Rep 10(1):13215
abstractText  There are currently no rapid, operant pain behaviors in rodents that use a self-report to directly engage higher-order brain circuitry. We have developed a pain detection assay consisting of a lick behavior in response to optogenetic activation of predominantly nociceptive peripheral afferent nerve fibers in head-restrained transgenic mice expressing ChR2 in TRPV1 containing neurons. TRPV1-ChR2-EYFP mice (n = 5) were trained to provide lick reports to the detection of light-evoked nociceptive stimulation to the hind paw. Using simultaneous video recording, we demonstrate that the learned lick behavior may prove more pertinent in investigating brain driven pain processes than the reflex behavior. Within sessions, the response bias of transgenic mice changed with respect to lick behavior but not reflex behavior. Furthermore, response similarity between the lick and reflex behaviors diverged near perceptual threshold. Our nociceptive lick-report detection assay will enable a host of investigations into the millisecond, single cell, neural dynamics underlying pain processing in the central nervous system of awake behaving animals.
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