| Primary Identifier | MGI:5014494 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(Pcp2-Prnp)E330Zbz |
| Strain of Origin | Not Specified | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Mice bearing this transgene express a truncated mouse prion protein (PRNP) lacking amino acids 32 through 121 under control of the regulatory elements of the Purkinje cell protein 2 (L7) gene. A cDNA encoding the modified PRNP and extending to the EcoR1 site in the 3' UTR was inserted into a cloned mouse genomic DNA segment containing the entire Pcp2 coding region, 4 kb of 5' flanking sequence and 2 kb of 3' flanking sequence. Insertion occurred at the BamH1 site in the fourth (third coding) exon, so the initial translation product has 80 amino acids of PCP2/L7 joined to its amino terminus; these presumably are removed with the PRNP N-terminal leader sequence. |
