| Primary Identifier | MGI:5140947 | Allele Type | Targeted |
| Gene | Unc93b1 | Transmission | Germline |
| Strain of Origin | 129P2/OlaHsd | Is Recombinase | false |
| Is Wild Type | false |
| description | ES cell line = EB3, derived from E14TG2a. EB3 cells carry one allele of Pou5f1 that has been inactivated via targeted integration of E14TG2a ES cells with an IRESBSDpA selection cassette. J:174308 does not mention whether the null allele of Pou5f1 is also present in the line of mice created. |
| molecularNote | Exon 2 was replaced with one in which an A to C mutation results in the amino acid substitution of alanine for aspartic acid at position 34 (D34A). A floxed neo cassette inserted downstream of exon 3 was removed by cre-mediated recombination. |
