| First Author | Xu J | Year | 2022 |
| Journal | STAR Protoc | Volume | 3 |
| Issue | 4 | Pages | 101907 |
| PubMed ID | 36595933 | Mgi Jnum | J:355077 |
| Mgi Id | MGI:7642093 | Doi | 10.1016/j.xpro.2022.101907 |
| Citation | Xu J, et al. (2022) Protocol to map multi-transmitter neurons in the mouse brain using intersectional strategy. STAR Protoc 3(4):101907 |
| abstractText | Although it is now known that certain neurons can produce, store, and release multiple neurotransmitters, their locations, abundance, and functions remain elusive. We developed intersectional genetic strategies to identify multi-transmitter neurons based on the expression of neurotransmitter-specific genes. Here we present our procedures for whole-brain mapping of GABA/glutamate co-releasing neurons. We also detail our technique for labeling GABA/glutamate neurons in specific brain regions with adeno-associated virus (AAV). Our protocol can be readily extended to other types of multi-transmitter neurons. For complete details on the use and execution of this protocol, please refer to Xu et al. (2022).(1). |
