Primary Identifier | MGI:5285428 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Mirc5 |
Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The entire 2 kb cluster including Mir290, Mir291a, Mir292, Mir291b, Mir293, Mir294, and Mir295 was replaced with a pgk-neo cassette via homologous recombination. RT-PCR confirmed the absence of mature micro RNAs in homozygous ES cells. |