| First Author | Wang B | Year | 2011 |
| Journal | Biochem Biophys Res Commun | Volume | 410 |
| Issue | 2 | Pages | 289-94 |
| PubMed ID | 21658371 | Mgi Jnum | J:174970 |
| Mgi Id | MGI:5141573 | Doi | 10.1016/j.bbrc.2011.05.134 |
| Citation | Wang B, et al. (2011) Generation of a Slc39a8 hypomorph mouse: markedly decreased ZIP8 Zn(2)/(HCO) transporter expression. Biochem Biophys Res Commun 410(2):289-94 |
| abstractText | Previously this laboratory has identified the mouse Slc39a8 gene encoding the ZIP8 transporter, important in cadmium uptake. ZIP8 functions endogenously as a electroneutral Zn(2+)/(HCO(3)(-))(2) symporter, moving both ions into the cell. The overall physiological importance of ZIP8 remains unclear. Herein we describe generation of a mouse line carrying the Slc39a8(neo) allele, containing the Frt-flanked neomycin-resistance (neo) mini-cassette in intron 3 and loxP sites in introns 3 and 6. Cre recombinase functions correctly in Escherichia coli and in adeno-Cre-infected mouse fetal fibroblasts, but does not function in the intact mouse for reasons not clear. Slc39a8(neo) is a hypomorphic allele, because Slc39a8(neo/neo) homozygotes exhibit dramatically decreased ZIP8 expression in embryo, fetus, and visceral yolk sac - in comparison to their littermate wild-type controls. This ZIP8 hypomorph will be instrumental in studying developmental and in utero physiological functions of the ZIP8 transporter. |
