| Primary Identifier | MGI:5285646 | Allele Type | Targeted |
| Attribute String | Humanized sequence | Gene | Myh9 |
| Inheritance Mode | Semidominant | Transmission | Germline |
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | The endogenous myosin, heavy polypeptide 9, non-muscle (Myh9) gene was modified by the introduction into exon 39 of a mutation resulting in replacement of glutamic acid with lysine at amino acid position 1841, in the rod region of the protein (E1841K), and by insertion of a loxP-flanked PGK-neor cassette into the preceding intron (intron 38). This modification replicates a mutation associated with a human MYH9-related disease (MYH9-RD). The selection cassette has been deleted by Cre recombinase-mediated excision. Immunoblot analysis demonstrates that levels of MYH9 protein are similar in lungs of homozygous mutant, heterozygous and wild-type mice. |
