| First Author | Liu C | Year | 2021 |
| Journal | Chin Med | Volume | 16 |
| Issue | 1 | Pages | 49 |
| PubMed ID | 34187513 | Mgi Jnum | J:333762 |
| Mgi Id | MGI:7440143 | Doi | 10.1186/s13020-021-00453-y |
| Citation | Liu C, et al. (2021) Honeysuckle-derived microRNA2911 inhibits tumor growth by targeting TGF-beta1. Chin Med 16(1):49 |
| abstractText | BACKGROUND: Honeysuckle is a time-honored herb with anticancer activity in traditional Chinese medicine. Recently, accumulating reports are suggesting that the microRNAs in this medicinal plant not only play a physiological role in their original system, but also can be transmitted to another species as potential therapeutic components. In the numerous bioactive investigations, the anti-tumor effects of these microRNAs in the magical herb are rarely studied, especially the special miR2911, a honeysuckle-encoded atypical microRNA, with high stability during the boiling process and unique biological activity to target TGF-beta1 mRNA. METHODS: Luciferase assay was conducted to test the ability of miR2911 to target TGF-beta1 mRNA. ELISA was performed to determine the expression level of TGF-beta1 of mouse colorectal adenocarcinoma CT26 cells when treated with miR2911 and tumor tissue in Sidt1(+/+) and Sidt1(-/-) mice. qRT-PCR was performed to examine the level of expression of miR2911. Tumor-bearing wild and nude mice were employed to evaluate the anti-tumor effect of honeysuckle and miR2911 in vivo. Tumor tissue necrosis was observed by H&E staining. Besides, the infiltration of T lymphocytes across solid tumors was tested by immunostaining staining. RESULTS: Our results showed that honeysuckle slowed the development of colon cancer down. Further research showed that miR2911 could bind strongly to TGF-beta1 mRNA and down-regulate the expression of TGF-beta1 and had a high stability under boiling and acid condition. Moreover, SIDT1 mediated dietary miR2911 inter-species absorption. And we found that miR2911 had a similar anticancer effect as honeysuckle. Mechanistically, miR2911 reversed the tumor-promoting effect of TGF-beta1 by an increase of T lymphocytes infiltration, resulting in slowing the colon cancer process in immunocompetent mice. Consistent with this inference, the anti-tumor effect of miR2911 was revealed to be abolished in T cell immune deficiency mice. CONCLUSION: Taken together, honeysuckle-derived miR2911 showed an anti-tumor effect in colon cancer through targeting TGF-beta1 mRNA. The down-regulation of TGF-beta1 promoted T lymphocytes infiltration, and accordingly impeded the colon tumor development. |
