Primary Identifier | MGI:5293612 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Gbe1 |
Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<b-m2> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | An FRT site was inserted upstream of exon 7 and an floxed pgk-neo cassette and FRT site were inserted downstream of exon 7 via homologous recombination. Flp mediated recombination deleted exon 7 and the neo cassette. Western blot analysis confirmed the absence of protein expression in muscle extracts from homozygous mice at E17.5. |