Primary Identifier | MGI:5295999 | Allele Type | Targeted |
Attribute String | Reporter | Gene | Sox2 |
Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | An construct containing the EGFP sequence with an Frt-flanked neo cassette was targeted to the Sox2 locus using homologous recombination in V6.5 ES cells. In correctly targeted clones, EGFP is expressed under control of the endogenous Sox2 regulatory elements. Germline knock-in mice were genearated and lines were established. |