| First Author | Harcha PA | Year | 2019 |
| Journal | Front Immunol | Volume | 10 |
| Pages | 2703 | PubMed ID | 31849935 |
| Mgi Jnum | J:298530 | Mgi Id | MGI:6480221 |
| Doi | 10.3389/fimmu.2019.02703 | Citation | Harcha PA, et al. (2019) Pannexin-1 Channels Are Essential for Mast Cell Degranulation Triggered During Type I Hypersensitivity Reactions. Front Immunol 10:2703 |
| abstractText | Mast cells (MCs) release pro-inflammatory mediators through a process called degranulation response. The latter may be induced by several conditions, including antigen recognition through immunoglobulin E (IgE) or "cross-linking," classically associated with Type I hypersensitivity reactions. Early in this reaction, Ca(2+) influx and subsequent increase of intracellular free Ca(2+) concentration are essential for MC degranulation. Several membrane channels that mediate Ca(2+) influx have been proposed, but their role remains elusive. Here, we evaluated the possible contribution of pannexin-1 channels (Panx1 Chs), well-known as ATP-releasing channels, in the increase of intracellular Ca(2+) triggered during cross-linking reaction of MCs. The contribution of Panx1 Chs in the degranulation response was evaluated in MCs from wild type (WT) and Panx1 knock out (Panx1(-/-)) mice after anti-ovalbumin (OVA) IgE sensitization. Notably, the degranulation response (toluidine blue and histamine release) was absent in Panx1(-/-) MCs. Moreover, WT MCs showed a rapid and transient increase in Ca(2+) signal followed by a sustained increase after antigen stimulation. However, the sustained increase in Ca(2+) signal triggered by OVA was absent in Panx1(-/-) MCs. Furthermore, OVA stimulation increased the membrane permeability assessed by dye uptake, a prevented response by Panx1 Ch but not by connexin hemichannel blockers and without effect on Panx1(-/-) MCs. Interestingly, the increase in membrane permeability of WT MCs was also prevented by suramin, a P2 purinergic inhibitor, suggesting that Panx1 Chs act as ATP-releasing channels impermeable to Ca(2+). Accordingly, stimulation with exogenous ATP restored the degranulation response and sustained increase in Ca(2+) signal of OVA stimulated Panx1(-/-) MCs. Moreover, opening of Panx1 Chs in Panx1 transfected HeLa cells increased dye uptake and ATP release but did not promote Ca(2+) influx, confirming that Panx1 Chs permeable to ATP are not permeable to Ca(2+). These data strongly suggest that during antigen recognition, Panx1 Chs contribute to the sustained Ca(2+) signal increase via release of ATP that activates P2 receptors, playing a critical role in the sequential events that leads to degranulation response during Type I hypersensitivity reactions. |
