| Primary Identifier | MGI:5302030 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Usp2 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A floxed neo cassette was inserted upstream of exon 3 and a loxP site was inserted downstream of exon 4 via homologous recombination. Cre mediated recombination removed the neo cassette and exons 3 - 4. Western blot analysis of total retina lysate from homozygous mice confirmed the absence of the 69 kDa isoform but did detect low levels of expression at about 45 kDa. RT_PCR analysis also detected low levels of expression of a short transcript. Sequencing determined this transcript was formed by alternative splicing of exon 1 to exon 5. |
