| Primary Identifier | MGI:5303388 | Allele Type | Targeted |
| Attribute String | Recombinase, Reporter | Gene | Hprt1 |
| Transmission | Cell Line | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | true | Is Wild Type | false |
| Project Collection | Pleiades Promoter Project |
| molecularNote | The Ple231-EGFP/Cre plasmid (pEMS1099) was created by inserting the Ple231 MiniPromoter (derived from specific promoter/enhancer/regulatory regions of the human troponin T type 1 (skeletal, slow) (TNNT1) gene) into the multiple cloning site of the pEMS1302 plasmid vector backbone. This placed the Ple231 MiniPromoter upstream of a minimal F5 mutant-frt site, a Kozak consensus sequence, an EGFP/Cre fusion gene (enhanced green fluorescent protein (EGFP; with mutated TAA to TTA stop) and Cre recombinase gene), a nuclear localization signal, a minimal wildtype frt site, an SV40 early polyA signal, a human HPRT complementary sequence (containing exon 1, intron 1, exon 2, and part of intron 2), a mouse 3' Hprt homology arm, an I-Sce linearization cut site, and a mouse 5' Hprt homology arm. This Ple231-EGFP/Cre construct was targeted as a single copy knock-in to the Hprtb-m3 mutant locus on the X Chromosome via electroporation into E14TG2a embryonic stem cells. |
