| Primary Identifier | MGI:5303302 | Allele Type | Targeted |
| Attribute String | Reporter | Gene | Hprt1 |
| Transmission | Cell Line | Strain of Origin | (B6.129P2-Hprt1<b-m3>/J x 129S-Gt(ROSA)26Sor<tm1Sor>/J)F1 |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | Pleiades Promoter Project |
| molecularNote | The Ple170-EGFP plasmid (pEMS1430) was created by inserting the Ple170 MiniPromoter (derived from specific promoter/enhancer/regulatory regions of the human pogo transposable element with ZNF domain (POGZ) gene) into the multiple cloning site of the pEMS1307 plasmid vector backbone. This placed the Ple170 MiniPromoter upstream of a full F5 mutant-frt site, a Kozak consensus sequence, an enhanced green fluorescent protein (EGFP; with mutated TAA to TTA stop), a tetracysteine residue, a nuclear localization signal, a full wildtype frt site, an SV40 early mRNA polyA signal, a human HPRT complementary sequence (containing exon 1, intron 1, exon 2, and part of intron 2), a mouse 3' Hprt homology arm, an I-Sce linearization cut site, and a mouse 5' Hprt homology arm. This Ple170-EGFP construct was targeted as a single copy knock-in to the Hprtb-m3 mutant locus on the X Chromosome via electroporation into mEMS1204 embryonic stem cells. |
