| First Author | Aguirrebengoa M | Year | 2021 |
| Journal | STAR Protoc | Volume | 2 |
| Issue | 4 | Pages | 101009 |
| PubMed ID | 34950888 | Mgi Jnum | J:358345 |
| Mgi Id | MGI:7712265 | Doi | 10.1016/j.xpro.2021.101009 |
| Citation | Aguirrebengoa M, et al. (2021) Purification of astrocyte subtype based on a double reporter mice approach for downstream transcription profiling. STAR Protoc 2(4):101009 |
| abstractText | Characterizing the molecular signature of a cell subtype leads to a better understanding of cell diversity, as this molecular data can identify new cellular markers and offer insights about cell function. Here, we describe an efficient protocol to separate a subtype of astrocytes, the Olig2-AS, from other glial cells by using a double reporter mouse approach and to determine the transcriptome profile of the Olig2-AS from the postnatal spinal cord using RNA-sequencing analysis. For complete details on the use and execution of this protocol, please refer to Ohayon et al. (2021). |
