Primary Identifier | MGI:5308794 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Mirc21 |
Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A loxP site was inserted upstream of Mir34b and an FRT-flanked neo cassette with a 3' loxP site was inserted downstream of Mir34c. Cre-mediated recombination removed the neo cassette and both microRNAs. Real-time PCR confirmed the absence of transcript expression in mouse embryonic fibroblasts. |