| First Author | Chiaruttini G | Year | 2016 |
| Journal | Cell Rep | Volume | 14 |
| Issue | 11 | Pages | 2624-36 |
| PubMed ID | 26972013 | Mgi Jnum | J:234824 |
| Mgi Id | MGI:5790919 | Doi | 10.1016/j.celrep.2016.02.055 |
| Citation | Chiaruttini G, et al. (2016) The SNARE VAMP7 Regulates Exocytic Trafficking of Interleukin-12 in Dendritic Cells. Cell Rep 14(11):2624-36 |
| abstractText | Interleukin-12 (IL-12), produced by dendritic cells in response to activation, is central to pathogen eradication and tumor rejection. The trafficking pathways controlling spatial distribution and intracellular transport of IL-12 vesicles to the cell surface are still unknown. Here, we show that intracellular IL-12 localizes in late endocytic vesicles marked by the SNARE VAMP7. Dendritic cells (DCs) from VAMP7-deficient mice are partially impaired in the multidirectional release of IL-12. Upon encounter with antigen-specific T cells, IL-12-containing vesicles rapidly redistribute at the immune synapse and release IL-12 in a process entirely dependent on VAMP7 expression. Consistently, acquisition of effector functions is reduced in T cells stimulated by VAMP7-null DCs. These results provide insights into IL-12 intracellular trafficking pathways and show that VAMP7-mediated release of IL-12 at the immune synapse is a mechanism to transmit innate signals to T cells. |
