| First Author | Fang W | Year | 1996 |
| Journal | Immunity | Volume | 4 |
| Issue | 3 | Pages | 291-9 |
| PubMed ID | 8624819 | Mgi Jnum | J:31931 |
| Mgi Id | MGI:79435 | Doi | 10.1016/s1074-7613(00)80437-9 |
| Citation | Fang W, et al. (1996) Frequent aberrant immunoglobulin gene rearrangements in pro-B cells revealed by a bcl-xL transgene. Immunity 4(3):291-9 |
| abstractText | During B lymphocyte development, pro-B cells that fail to rearrange an immunoglobulin heavy (IgH) chain allele productively are thought to undergo developmental arrest and death, but because these cells are short-lived in vivo they are not well characterized. Transgenic mice expressing the apoptosis regulatory gene bcl-xL in the B lineage developed large expansions of pro-B cells in bone marrow. V(D)J rearrangements in the expanded populations were nearly all nonproductive, and DJH rearrangements were enriched for joints in DH reading frame 2 and for aberrant joints with extensive DH or JH deletions. Thus, the death of pro-B cells with failed immunoglobulin rearrangements occurs by apoptosis, and bcl-xL can deliver a strong survival signal at the pro-B stage. This analysis also demonstrated that immunoglobulin gene rearrangement is less precise than previously appreciated. |
