| Primary Identifier | MGI:5314516 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(EEF1A1-Socs6)2Pwg |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| description | This is one of two transgenic lines that were generated and characterized. The other, Tg(EEF1A1-Socs6)1Pwg, expresses the transgene at 5-fold the level at which wild-type mice express the endogenous gene. (J:181312) |
| molecularNote | A full length mouse Sox6 cDNA modified to append a human MYC tag to the carboxyl terminus of the protein product was cloned into the pEFneo vector downstream of the human eukaryotic elongation factor 1 alpha 1 promoter. Northern blot analysis using a Socs6 cDNA probe and western blot analysis with an anti-MYC antibody demonstrate that mice of line 2 express transgene-derived mRNA and protein, respectively, at 10-fold the levels expressed by wild-type mice from the endogenous gene in liver, muscle and T-lymphocytes. |
