| Primary Identifier | MGI:5314752 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Car5b |
| Transmission | Germline | Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Thirty bp (codons for 10 amino acids) in exon 3 were replaced with a floxed cassette composed of a neomycin resistance gene and cre placed under the direction of a testis specific angiotensin converting enzyme pomoter. Cre expression in the germline of chimeric male mice excised the inserted cassette leaving a deletion in exon 3 including two of three zinc binding histidine residues and introducing a frameshift beginning in exon 4. A stop codon was created after 11 missence amino acids. |
