Primary Identifier | MGI:5317216 | Allele Type | Transgenic |
Attribute String | Inducible, Recombinase, Reporter | Gene | Tg(Lfng-TagRFP/cre/ERT2)8Amc |
Strain of Origin | (C57BL/6 x DBA)F1 | Induced With | tamoxifen |
Is Recombinase | true | Is Wild Type | false |
molecularNote | A targeting construct containing the Tag-RFPT variant of Red Fluorescent Protein and Frt site flanked Kanamycin/neomycin selection cassette was inserted into the consensus start ATG codon of the mouse Lfng gene in a BAC. The PGK-Neo/Kan cassette was removed by transient Flpe-recombinase expression. The resulting BAC transgene was microinjected into (C57BL/6 x DBA) F1 donor eggs. Founder line 8 was subsequently established. |