Primary Identifier | MGI:5320058 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Mir185 |
Transmission | Cell Line | Strain of Origin | C57BL/6N-A<tm1Brd> |
Is Recombinase | false | Is Wild Type | false |
Project Collection | Sanger miRNA knockouts |
molecularNote | The insertion of the PGK_EM7_PuDtk_bGHpA cassette created a deletion of size 415 starting at position 18058567 and ending at position 18058982 of Chromosome 16 (Build GRCm39). This deletion eliminates the DNA sequence for this microRNA. The cassette is composed of a loxP site followed by an F3 site followed by a PGK-puromycin-delta-tk cassette, a loxP site and finally an FRT site. |