| Primary Identifier | MGI:5320065 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Mir499 |
| Transmission | Cell Line | Strain of Origin | C57BL/6N-A<tm1Brd> |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | Sanger miRNA knockouts |
| molecularNote | The insertion of the PGK_EM7_PuDtk_bGHpA cassette created a deletion of size 3424 starting at position 155330353 and ending at position 155333777 of Chromosome 2 (Build GRCm39). This deletion eliminates the DNA sequence for this microRNA. The cassette is composed of a loxP site followed by an F3 site followed by a PGK-puromycin-delta-tk cassette, a loxP site and finally an FRT site. |