Primary Identifier | MGI:5320061 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Mir350 |
Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
Is Recombinase | false | Is Wild Type | false |
Project Collection | Sanger miRNA knockouts |
molecularNote | The insertion of the PGK_EM7_PuDtk_bGHpA cassette created a deletion of size 216 starting at position 176599829 and ending at position 176600045 of Chromosome 1 (Build GRCm39). This deletion eliminates the DNA sequence for this microRNA. The cassette is composed of a loxP site followed by an F3 site followed by a PGK-puromycin-delta-tk cassette, a loxP site and finally an FRT site. |