Primary Identifier | MGI:5320075 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Mir25 |
Transmission | Cell Line | Strain of Origin | C57BL/6N-A<tm1Brd> |
Is Recombinase | false | Is Wild Type | false |
Project Collection | Sanger miRNA knockouts |
molecularNote | The insertion of the PGK_EM7_PuDtk_bGHpA cassette created a deletion of size 591 starting at position 138163554 and ending at position 138164145 of Chromosome 5 (Build GRCm39). This deletion eliminates the DNA sequence for this microRNA. The cassette is composed of a loxP site followed by an F3 site followed by a PGK-puromycin-delta-tk cassette, a loxP site and finally an FRT site. |