Primary Identifier | MGI:5320152 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Mir210 |
Transmission | Germline | Strain of Origin | C57BL/6N |
Is Recombinase | false | Is Wild Type | false |
Project Collection | Sanger miRNA knockouts |
description | Cell line mirKO_ES_PuDtk_1A1 was successfully used to make chimeric mice. Germline transmission was accomplished. J:205868 |
molecularNote | The insertion of the PGK_EM7_PuDtk_bGHpA cassette created a deletion of size 237 starting at position 140801235 and ending at position 140801472 of Chromosome 7 (Build GRCm39). This deletion eliminates the DNA sequence for this microRNA. The cassette is composed of a loxP site followed by an F3 site followed by a PGK-puromycin-delta-tk cassette, a loxP site and finally an FRT site. |