| Primary Identifier | MGI:5426507 | Allele Type | Targeted |
| Attribute String | Dominant negative, Inserted expressed sequence, Reporter | Gene | Gt(ROSA)26Sor |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Homologous recombination in E14 ES cells introduced a dominant negative Mastermind-like 1/EGFP fusion sequence ((Maml1/EGFP) into the Gt(ROSA)26Sor locus. An Frt-flanked neo cassette was also introduced with the targeting construct, and was subsequently deleted from germline chimeras by breeding them with Flpe-expressing mice. The dnMaml1 fusion protein is a potent inhibitor of the 4 mammalian Notch receptors. |
