| Primary Identifier | MGI:5426519 | Allele Type | Transgenic |
| Attribute String | Inducible, Recombinase, Transactivator | Gene | Tg(Col2a1/Acan-rtTA,tetO-cre)#Vlf |
| Strain of Origin | Not Specified | Induced With | doxycycline/tetracycline |
| Is Recombinase | true | Is Wild Type | false |
| molecularNote | A bigenic construct was generated with a 0.9kb fragment of the Col2a1 promoter, 4 tandem copies of a cartilage-specific Acan enhancer in an intron, a splice acceptor site, the reverse tetracycline transactivator coding sequence and a polyadenylation site composing the first gene. A 4 kb Trp53 intron insulator sequence separated the rtTA sequence from the second part of the construct, consisting of a heptad of tandem copies of a tetracycline-responsive element (tetO), the human cytomegalovirus promoter (hCMV), the cre recombinase coding sequence, and a polyadenylation site. No founder information for the transgene is available. Upon treatment of transgenic mice with doxycycline, the rtTA is activated and binds to tetO to induce cre expression. |
