| First Author | Li Z | Year | 2022 |
| Journal | Sci Bull (Beijing) | Volume | 67 |
| Issue | 4 | Pages | 408-426 |
| PubMed ID | 36546093 | Mgi Jnum | J:354569 |
| Mgi Id | MGI:7735041 | Doi | 10.1016/j.scib.2021.11.013 |
| Citation | Li Z, et al. (2022) Single-cell RNA-seq and chromatin accessibility profiling decipher the heterogeneity of mouse gammadelta T cells. Sci Bull (Beijing) 67(4):408-426 |
| abstractText | The distinct characteristics of gammadelta T cells determine their vital roles in the formation of local immune responses and contribute to tissue homeostasis. However, the heterogeneity of gammadelta T cells across tissues remains unclear. By combining transcriptional and chromatin analyses with a truly unbiased fashion, we constructed a single-cell transcriptome and chromatin accessibility landscape of mouse gammadelta T cells in the lymph, spleen, and thymus. We also revealed the heterogeneity of gammadelta T1 and gammadelta T17 cells across these tissues and inferred their potential regulatory mechanisms. In the thymus, we reconstructed the developmental trajectory and gained further insights into the signature genes from the mature stage, intermediate stage, and immature stage of gammadelta T cells on the basis of single-cell RNA sequencing and single-cell assay for transposase-accessible chromatin sequencing data. Notably, a novel Gzma(+) gammadelta T cell subset was identified with immature properties and only localized to the thymus. Finally, NR1D1, a circadian transcription factor (TF), was validated as a key and negative regulator of gammadelta T17 cell differentiation by performing a combined analysis of TF motif enrichment, regulon enrichment, and Nr1d1 knockout mice. In summary, our data represent a comprehensive mapping on the transcriptome and chromatin accessibility dynamics of mouse gammadelta T cells, providing a valuable resource and reference for future studies on gammadelta T cells. |
