| Primary Identifier | MGI:5431129 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Zc3hc1 |
| Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site was inserted upstream of exon 1 and an FRT flanked neo cassette and loxP site were inserted downstream of exon 2 via homologous recombination. Recombinase mediated recombination removed the neo cassette and exons 1 and 2. RT-PCR and western blot analysis confirmed the absence of expression in homozygous MEFs and testicular cells. |
