| Primary Identifier | MGI:5431529 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Rnaseh2b |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exon 7 was replaced with a modified exon 7 in which a G to A point mutation results in the amino acid substitution of threonine for alanine at position 174 (A174T). An additional mutation produces a G to T transversion resulting in a stop codon at position 202 (E202X). A floxed neo cassette was inserted upstream of the modified exon 7. Western blot analysis confirmed the absence of protein expression in E9.5 embryos. |
