| First Author | Higashitsuji H | Year | 2020 |
| Journal | Biochem Biophys Res Commun | Volume | 533 |
| Issue | 4 | Pages | 1191-1197 |
| PubMed ID | 33041006 | Mgi Jnum | J:313315 |
| Mgi Id | MGI:6705428 | Doi | 10.1016/j.bbrc.2020.10.004 |
| Citation | Higashitsuji H, et al. (2020) Mammalian cold-inducible RNA-binding protein facilitates wound healing through activation of AMP-activated protein kinase. Biochem Biophys Res Commun 533(4):1191-1197 |
| abstractText | The skin is usually maintained within a temperature range that induces cold-inducible RNA-binding protein (Cirp). To determine whether Cirp plays a role in barrier function of the skin, we analyzed the skin wound healing in cirp-knockout (KO) mice. They exhibited delayed wound healing compared with wild-type littermates in the absence as well as presence of skin contraction. Dermal fibroblasts and keratinocytes from cirp-KO mice migrated slower than those from wild-type mice. When expression of Cirp was downregulated in cultured cells, migration rate was decreased. Cirp bound liver-kinase-B1 (LKB1) in the nucleus and was suggested to enhance its translocation to the cytoplasm, resulting in enhanced phosphorylation of AMP-activated protein kinase (AMPK) and cell motility. Stimulation of AMPK ameliorated the delayed wound healing in cirp-KO mice. These findings suggest that Cirp facilitates skin wound healing by enhancing cell migration via AMPK, indicating roles for Cirp in linking skin temperature with metabolism and defense mechanism. |
