| Primary Identifier | MGI:5433373 | Allele Type | Targeted |
| Attribute String | Inducible, Recombinase | Gene | Axin2 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Induced With | tamoxifen | Is Recombinase | true |
| Is Wild Type | false |
| molecularNote | A cre/ERT2 sequence (cre recombinase fused to a G400V/M543A/L544A triple mutation ofthe human estrogen receptor ligand binding domain) and an FRT-flanked puromycin resistance cassette were knocked into exon 2 of the Axin2 gene. Transient flp expression in ES cells and crosses to flp-expressing strains were unsuccessful at excising the selection cassette, but its presence does not seem to have negative side effects. |
