| Primary Identifier | MGI:5433857 | Allele Type | Targeted |
| Attribute String | Humanized sequence, Null/knockout | Gene | Slc4a4 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv) |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A floxed neo cassette was inserted into intron 10 and a G to A mutation, resulting in a premature stop codon at position 516 (W516X), was inserted into exon 11 via homologous recombination. Cre mediated recombination removed the neo cassette. RT-PCR indicates a significant reduction in expression in kidneys in homozygous and heterozygous mice. Immunofluorescence analysis failed to detect protein expression in kidneys from homozygous mice. |
