Primary Identifier | MGI:5435691 | Allele Type | Transgenic |
Attribute String | Inducible, Recombinase, Reporter | Gene | Tg(Wnt11-TagRFP/cre/ERT2)28Amc |
Strain of Origin | (C57BL/6 x DBA)F1 | Induced With | tamoxifen |
Is Recombinase | true | Is Wild Type | false |
molecularNote | A targeting construct containing a myristoylated Tag-RFPT variant of Red Fluorescent Protein, cre/ERT2, and FRT site flanked Kanamycin/neomycin selection cassette was inserted into the consensus start ATG codon of the mouse Wnt11 gene in the RP23-122D14 BAC. The PGK-Neo/Kan cassette was removed by transient Flpe-recombinase expression. The resulting modified Wnt11-myrTagRFP-IRES-CE BAC transgene was microinjected into (C57BL/6 x DBA) F1 donor eggs. Founder line 28 was subsequently established. |