Primary Identifier | MGI:5435947 | Allele Type | Targeted |
Attribute String | Recombinase, RMCE-ready | Gene | Etl4 |
Strain of Origin | (C57BL/6NCrlj x CBA/JNCrlj)F1 | Is Recombinase | true |
Is Wild Type | false |
molecularNote | Cre recombinase was inserted into intron 14 of the Etl4 (Skt) locus by recombinase mediated cassette exchange using an exchangeable gene trap system using left element/right element (LE/RE) mutant lox sites. The IRES-cre sequence was exchanged with the IRES-lacZ-neo unit in the original trap vector by co-electroporation of the vector and pCAGGS-cre into TT2 ES cells containing the trapped Etl4 gene. |