| Primary Identifier | MGI:5438324 | Allele Type | Targeted |
| Attribute String | Conditional ready, Null/knockout | Gene | Pkhd1 |
| Transmission | Germline | Strain of Origin | 129 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The targeting vector was designed with a loxP-STOP-loxP (LSL) cassette containing a puromycin acetyltransferase gene (pac) and 4 copies of the SV40 viral transcriptional termination sequence inserted into intron 2 and two SV5-Pk epitope tags (26 amino acids long) inserted in-frame into exon 3. The LSL cassette terminates all transcripts in intron 2 generating a null allele. Northern blotting and RT-PCR analysis confirmed the absence of mRNA. |
