| First Author | Katada Y | Year | 2023 |
| Journal | Mol Ther Methods Clin Dev | Volume | 30 |
| Pages | 1-13 | PubMed ID | 37324975 |
| Mgi Jnum | J:347117 | Mgi Id | MGI:7616641 |
| Doi | 10.1016/j.omtm.2023.05.011 | Citation | Katada Y, et al. (2023) Starburst amacrine cells amplify optogenetic visual restoration through gap junctions. Mol Ther Methods Clin Dev 30:1-13 |
| abstractText | Ectopic induction of optogenetic actuators, such as channelrhodopsin, is a promising approach to restoring vision in the degenerating retina. However, the cell type-specific response of ectopic photoreception has not been well understood. There are limits to obtaining efficient gene expression in a specifically targeted cell population by a transgenic approach. In the present study, we established a murine model with high efficiency of gene induction to retinal ganglion cells (RGCs) and amacrine cells using an improved tetracycline transactivator-operator bipartite system (KENGE-tet system). To investigate the cell type-specific visual restorative effect, we expressed the channelrhodopsin gene into RGCs and amacrine cells using the KENGE-tet system. As a result, enhancement in the visual restorative effect was observed to RGCs and starburst amacrine cells. In conclusion, a photoresponse from amacrine cells may enhance the maintained response of RGCs and further increase or improve the visual restorative effect. |
