| Primary Identifier | MGI:5440446 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Ngb |
| Transmission | Germline | Strain of Origin | 129S2/SvPas |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site was inserted upstrea of exon 2 and an FRT flanked neo cassette and loxP site were introduced downstream of exon 3 via homologous recombination. Recombinase mediated recombination removed exons 2 and 3 and the neo cassette. RT-QPCR, IHC and western blot analyses confirmed the absence of mRNA and protein expression in homozygous mice. |
