| Primary Identifier | MGI:5443888 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change | Gene | Col1a1 |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| description | ES cells contain Cdkn2atm1.1Brn Nf2tm2Gth Trp53tm1Brn in addition to the targeted Col1a1tm3Nki allele. |
| molecularNote | FVB.129P2-Cdkn2atm1.1Brn Nf2tm2Gth Trp53tm1Brn Col1a1tm3Nki ES cells were retargeted to replace the frt-flanked PGK-Neo cassette in the 3' UTR of the Col1a1 by a "Flp-in" plasmid encoding a frt-lox66-Cag-lox71-Luc2 inversion transgene, called inv-Cag-Luc2, by co-transfected with a Flpe-expressing plasmid to facilitate recombination into the 3' UTR of the Col1a1 locus. This transgene contains the constitutive CAGGs promoter that drives the expression of the Hygromycin-resistance gene, present in the targeted Col1a1 locus. Upon expression of the Cre recombinase, the promoter is inverted due to flanking mutant loxP sites, i.e. lox66 and lox71, placing the promoter in front of the firefly Luciferase2 gene. |
