| Primary Identifier | MGI:5444208 | Allele Type | Transgenic |
| Attribute String | Dominant negative, Humanized sequence, Inserted expressed sequence | Gene | Tg(ACTA1-TPM3*M9R)4Hrd |
| Strain of Origin | FVB/NJ | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | First, the human tropomyosin 3 gene was used to isolate a full-length alphaTmslow cDNA sequence. Next, the sequence was modified by site-directed mutagenesis to have the ATG-to-AGG base pair substitution encoding a methionine-to-arginine substitution at codon 9 (M9R); resulting in the dominant-negative alphaTmslow(Met9Arg) mutation associated with human nemaline myopathy. The mutant cDNA sequence was placed downstream of a 2.2 kbp human alpha-skeletal actin (ACTA1) promoter sequence, and upstream of a 1022 bp cassette containing the SV40 small t antigen intron and 3' untranslated region. Lines 4, 9 and 14 were generated with high, low and moderate expression levels, respectively. |
