| Primary Identifier | MGI:5461560 | Allele Type | Targeted |
| Gene | Ebf2 | Transmission | Germline |
| Strain of Origin | Not Specified | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | A construct containing a full length cDNA of Zfp423 with a single nucleotide insertion (3877_3878insT) between ZF 27 and ZF 28, corresponding to amino acid 1208 of the protein, followed by an IRES, YFP and a floxed neo selection cassette was inserted into the 5' UTR, replacing the first five exons of the locus. The insertion creates a termination codon and altered reading frame in all reading frames. Cre mediated recombination removed the neo cassette. Western blot analysis confirmed the expression of both inserted proteins. |
