Primary Identifier | MGI:5464892 | Allele Type | Targeted |
Attribute String | Not Specified | Gene | Ndufs4 |
Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
Is Recombinase | false | Is Wild Type | false |
description | ES cell = PC1 (129S6/SvEvTac) |
molecularNote | A point mutant construct with a floxed neo cassette created a 1-base deletion causing a frameshift and premature stop codon in the last exon upstream of a conserved 10 to 15 amino acid C-terminal sequence. Western blot analysis confirmed reduced expression of a truncated protein in heterozygous mice. |