| Primary Identifier | MGI:5469976 | Allele Type | Targeted |
| Attribute String | Humanized sequence, Inserted expressed sequence, Null/knockout | Gene | Cbfb |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exon 5 was replaced by a construct composed of the first 25 bp of mouse exon 5 fused to the 3' end of a human CBFB/MYH11 cDNA. This fusion protein is produced by a chromosome 16 inversion in patients with acute myeloid leukemia subtype M4Eo. The human cDNA fragment used contained the last 66 bp of human exon 5 and exons 33-42 of MYH11 lacking the sequence encoding amino acids the last 95 amino acids (deltaC95). A bovine growth hormone polyadenylation site and a floxed PGK-neomycin cassette were inserted immediately downstream. |
