Primary Identifier | MGI:5470177 | Allele Type | Transgenic |
Attribute String | Reporter | Gene | Tg(Gast-EGFP)1Tcw |
Strain of Origin | (C57BL/6 x CBA)F1 | Is Recombinase | false |
Is Wild Type | false |
molecularNote | The ENSEMBL 129S7/AB2.2-derived mouse genomic BAC clone bMQ262f21, encoding full-length gastrin, was modified to express EGFP from the Gast promoter. The open reading frame after the ATG start codon located in exons 2 and 3 was replaced by an EGFP-FRT-PGK-neomycin-FRT cassette. The PGK-neomycin cassette was removed via transfection with an arabinose-induced FLP recombinase plasmid. Line 1 was generated. |