| Primary Identifier | MGI:5471313 | Allele Type | Targeted |
| Attribute String | Inducible, Recombinase | Gene | Msi1 |
| Transmission | Germline | Strain of Origin | (C57BL/6 x 129)F1 |
| Induced With | tamoxifen | Is Recombinase | true |
| Is Wild Type | false |
| description | ES cells = B6/129 F1 |
| molecularNote | Sequences of the first exon downstream of the initial ATG of Msi1 in a BAC clone (RP23-268H8) were replaced with a cre/ERT2 cassette, poly A signal and Frt-flanked PGK-neo, by recombineering in bacterial cells. The targeting construct was transfected into B6/129 F1 ES cells. Targeted ES clones were injected into C57BL/6 blastocysts to generate chimeras. Germline mutants were bred to FLPe mice to remove the neo selection cassette. RT-PCR demonstrated that no transcript containing exon 1 is produced from the mutated allele. |
