| First Author | Thomas CM | Year | 2014 |
| Journal | Am J Physiol Heart Circ Physiol | Volume | 307 |
| Issue | 7 | Pages | H1036-45 |
| PubMed ID | 25085967 | Mgi Jnum | J:218108 |
| Mgi Id | MGI:5616680 | Doi | 10.1152/ajpheart.00340.2014 |
| Citation | Thomas CM, et al. (2014) Cardiac-specific suppression of NF-kappaB signaling prevents diabetic cardiomyopathy via inhibition of the renin-angiotensin system. Am J Physiol Heart Circ Physiol 307(7):H1036-45 |
| abstractText | Activation of NF-kappaB signaling in the heart may be protective or deleterious depending on the pathological context. In diabetes, the role of NF-kappaB in cardiac dysfunction has been investigated using pharmacological approaches that have a limitation of being nonspecific. Furthermore, the specific cellular pathways by which NF-kappaB modulates heart function in diabetes have not been identified. To address these questions, we used a transgenic mouse line expressing mutated IkappaB-alpha in the heart (3M mice), which prevented activation of canonical NF-kappaB signaling. Diabetes was developed by streptozotocin injections in wild-type (WT) and 3M mice. Diabetic WT mice developed systolic and diastolic cardiac dysfunction by the 12th week, as measured by echocardiography. In contrast, cardiac function was preserved in 3M mice up to 24 wk of diabetes. Diabetes induced an elevation in cardiac oxidative stress in diabetic WT mice but not 3M mice compared with nondiabetic control mice. In diabetic WT mice, an increase in the phospholamban/sarco(endo)plasmic reticulum Ca(2+)-ATPase 2 ratio and decrease in ryanodine receptor expression were observed, whereas diabetic 3M mice showed an opposite effect on these parameters of Ca(2+) handling. Significantly, renin-angiotensin system activity was suppressed in diabetic 3M mice compared with an increase in WT animals. In conclusion, these results demonstrate that inhibition of NF-kappaB signaling in the heart prevents diabetes-induced cardiac dysfunction through preserved Ca(2+) handling and inhibition of the cardiac renin-angiotensin system. |
