| First Author | Ord T | Year | 2012 |
| Journal | Cell Immunol | Volume | 280 |
| Issue | 1 | Pages | 68-75 |
| PubMed ID | 23261831 | Mgi Jnum | J:192615 |
| Mgi Id | MGI:5465495 | Doi | 10.1016/j.cellimm.2012.11.011 |
| Citation | Ord T, et al. (2012) Trib3 is regulated by IL-3 and affects bone marrow-derived mast cell survival and function. Cell Immunol 280(1):68-75 |
| abstractText | Mast cells are the principal effectors of IgE-mediated immune responses, including allergic reactions. Tribbles homolog 3 (Trib3) encodes a pseudokinase implicated in the cellular stress response and has been linked to inflammation in certain situations. Here we report the role of Trib3 in mouse bone marrow-derived mast cells (BMMCs). Our results show that Trib3 mRNA expression in BMMCs is positively regulated by the growth factor interleukin (IL)-3. BMMCs originating from Trib3 knockout mice demonstrate unaltered differentiation kinetics and cell surface expression of mast cell markers. When challenged with transient IL-3 deprivation, Trib3-deficient BMMCs display delayed recovery, and during prolonged IL-3 starvation, cell death is accelerated in Trib3-null cultures. IgE-dependent and pharmacologically induced degranulation is impaired in Trib3-deficient BMMCs, as is activation-induced cytokine mRNA expression. Thus, Trib3 contributes to the survival and activity of primary cultured mast cells, which suggests a role for Trib3 in the modulation of the immune response. |
